Journal: Nanoscale Advances

Article Title: Continuously manufactured single-core iron oxide nanoparticles for cancer theranostics as valuable contribution in translational research

doi: 10.1039/d0na00343c

Figure Lengend Snippet: Determination of cell viability of brain microvascular endothelial cells (hCMEC) after treatment with MNP samples (exemplarily T1). Cells were seeded on 96-well plates and treated with several concentrations of nanoparticles for various time periods. Cells were washed and thereafter incubated with a CCK-8 substrate. After 60 minutes, the absorbance was measured at λ = 450 nm. Untreated cells (Ctrl) were set to 100% viability. No cytotoxic effects were observed neither short term (4 h) (top) nor long-term up to 72 h (bottom).

Article Snippet: Immortalized human cerebral microvascular endothelial cells (hCMEC/D3; Biozol) were maintained in rat tail collagen-I (50 μg mL −1 ; Ibidi GmbH) -coated culture flasks in ECBM MV cell culture media (PromoCell) supplemented with 15% fetal bovine serum (FBS), 2.5 ng mL −1 basal fibroblast growth factor, 10 μg mL −1 sodium heparin (all Sigma-Aldrich).

Techniques: Incubation, CCK-8 Assay

Journal: Nanoscale Advances

Article Title: Continuously manufactured single-core iron oxide nanoparticles for cancer theranostics as valuable contribution in translational research

doi: 10.1039/d0na00343c

Figure Lengend Snippet: Determination of cell death and apoptosis of brain microvascular endothelial cells after treatment with MNPs for 24 h (top) and 72 h (bottom). Cells were analyzed by flow cytometry after staining with annexin-V-FITC and propidium iodide. Cell populations in percent of total cells have been blotted. One-way ANOVA followed by Tukey's multiple comparisons test was performed. The data of each group (living, dead, and apoptotic) were statistically evaluated separately from each other ( n = 3–4).

Article Snippet: Immortalized human cerebral microvascular endothelial cells (hCMEC/D3; Biozol) were maintained in rat tail collagen-I (50 μg mL −1 ; Ibidi GmbH) -coated culture flasks in ECBM MV cell culture media (PromoCell) supplemented with 15% fetal bovine serum (FBS), 2.5 ng mL −1 basal fibroblast growth factor, 10 μg mL −1 sodium heparin (all Sigma-Aldrich).

Techniques: Flow Cytometry, Staining